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To experimentally validate our hypothesis, we performed RNA fluorescence in situ hybridization (FISH) and immunofluorescence (IF) staining for GDIL and XRN2, respectively, in parental and resistant conditions

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The verification of Yl CRC1 disruption was performed by colony PCR, amplifying the genomic region targeted by the two gRNAs and comparing the amplicons length of the transformants, with that obtained from the parental strains

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GSH and Cys play essential roles in helping pepper mitigate Cd toxicity and promote normal growth under Cd stress, GSH mainly plays a role in the leaves, while Cys plays an important role in the fruits